December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Electrophysiologic Evidence of Functional Neuroprotection of Photoreceptors by X-Linked Inhibitor of Apoptosis (XIAP) in a Chemotoxic Model of Retinal Degeneration
Author Affiliations & Notes
  • SG Coupland
    Eye Institute University of Ottawa Ottawa ON Canada
  • D Petrin
    Children's Hospital of Eastern Ontario Research Institute Ottawa ON Canada
  • A Baker
    Children's Hospital of Eastern Ontario Research Institute Ottawa ON Canada
  • P Liston
    Children's Hospital of Eastern Ontario Research Institute Ottawa ON Canada
  • A Timmers
    University of Florida College of Medicine Gainesville FL
  • WW Hauswirth
    University of Florida College of Medicine Gainesville FL
  • Footnotes
    Commercial Relationships   S.G. Coupland, None; D. Petrin, None; A. Baker, None; P. Liston, None; A. Timmers, None; W.W. Hauswirth, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4605. doi:
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      SG Coupland, D Petrin, A Baker, P Liston, A Timmers, WW Hauswirth; Electrophysiologic Evidence of Functional Neuroprotection of Photoreceptors by X-Linked Inhibitor of Apoptosis (XIAP) in a Chemotoxic Model of Retinal Degeneration . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4605.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:. To assess functional neuroprotection from N-methyl-N-nitrosourea (MNU) induced retinal degeneration by XIAP, an X-linked inhibitor of apoptosis. Methods:At 6 weeks of age, thirty male Sprague-Dawley rats had a sub-retinal injection in one eye of adeno-associated virus encoding XIAP. At 6 weeks post-injection the animals received intra-peritoneal injection of 60 mg/kg of MNU, a DNA methylating agent. Flash electroretinograms (ERGs) were recorded from dark adapted eyes using DTL-fiber electrodes over an intensity range of –3.0 to 1.4 log cd.s/m2. ERGs were recorded at pre-treatment baseline and at 24, 48, 72 hours and 1 week post-MNU injection. Results:By 24 hours post-MNU injection the b-wave was significantly diminished in amplitude and delayed in both control and treated eyes (p<0.003); however, in 50% of animals the XIAP injected eye had significantly greater amplitude. By 1 week post-MNU injection the ERG was extinguished in all control eyes; whereas, an ERG was present in 33% of XIAP injected eyes. Conclusion:These results suggest that XIAP offers functional neuroprotection against this potent chemotoxic agent.

Keywords: 396 electroretinography: non-clinical • 419 gene transfer/gene therapy • 554 retina 
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