December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
SIV-mediated Gene Transfer Into Rat Retina
Author Affiliations & Notes
  • H Conrath
    Laboratoire de Therapie Genique CHU Hotel-Dieu Nantes France
  • G Duisit
    Laboratoire de Therapie Genique CHU Hotel-Dieu Nantes France
  • S Saleun
    Laboratoire de Therapie Genique CHU Hotel-Dieu Nantes France
  • Y Cherel
    Laboratoire d'Anatomie Pathologique Ecole Veterinaire de Nantes Nantes France
  • P Moullier
    Laboratoire de Therapie Genique CHU Hotel-Dieu Nantes France
  • F Rolling
    Laboratoire de Therapie Genique CHU Hotel-Dieu Nantes France
  • Footnotes
    Commercial Relationships   H. Conrath, None; G. Duisit, None; S. Saleun, None; Y. Cherel, None; P. Moullier, None; F. Rolling, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4608. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      H Conrath, G Duisit, S Saleun, Y Cherel, P Moullier, F Rolling; SIV-mediated Gene Transfer Into Rat Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4608.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To evaluate lentiviral vectors mediated rat retinal transduction using SIV pseudotyped with envelope proteins from vesicular stomatitis virus (VSV-G), lyssavirus (MK-G) or 4070A amphotropic MLV. The three pseudotyped lentivirus vectors carried CMV-driven Green Fluorescent Protein (GFP) or ß-Galactosidase (ß-Gal) reporter genes. Methods: Intravitreal and subretinal injections of recombinant SIV were performed in cohorts of wistar rats. GFP and ß-Gal expression were respectively evaluated by direct in vivo retinal imaging and X-Gal staining on paraffin sections. Since VSV-G-pseudotyped SIV vectors were concentrated before use, possible pseudotranduction was evaluated by producing empty SIV particles in the presence of a CMV-GFP plasmid lacking the encapsidation signal. Results: No transgene expression was detected after intravitreal injection of SIV vectors. However, selective transduction of RPE cells was repeatedly obtained after transscleral-transchoroïdal subretinal delivery of VSV-G, MK-G and 4070A pseudotyped SIV. GFP expression was maximum as soon as 2-3 days post administration and up to 5 months with no evidence for pseudotransduction. Conclusions:VSVG, lyssavirus, and 4070A amphotropic pseudotyped lentiviral vectors specifically target RPE cells with early onset of transgene expression. This vector holds promise as gene therapy vector for retinal degenerative disease originating in RPE cells.

Keywords: 419 gene transfer/gene therapy • 554 retina • 567 retinal pigment epithelium 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.