December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effect of Antisense against c-met/Hepatocyte Growth Factor receptor
H Kim; SC Park; Y Kim; J Suh
Author Affiliations & Notes
  • H Kim
    Ophthalmology College of Medicine The Catholic University of Korea Seoul Republic of Korea
  • SC Park
    Ophthalmology College of Medicine The Catholic University of Korea Seoul Republic of Korea
  • Y Kim
    Ophthalmology College of Medicine The Catholic University of Korea Seoul Republic of Korea
  • J Suh
    Ophthalmology College of Medicine The Catholic University of Korea Seoul Republic of Korea
  • Footnotes
    Commercial Relationships   H. Kim, None; S.C. Park, None; Y. Kim, None; J. Suh, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4627. doi:
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      H Kim, SC Park, Y Kim, J Suh; Effect of Antisense against c-met/Hepatocyte Growth Factor receptor . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4627.

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Abstract

Abstract: : Purpose: To determine the effect of phosphorothioate antisense oligonucletides directed against c-met/HGF receptor on RPE cell differentiation and proliferation. Methods: We transfected cultured human RPE with c-met-specific phosphorothioate antisense oligonucleotides that were designed as 18-mer sequences complementary to c-met mRNA. Target mRNA was quantified by RNase protection assay. Cellular differentiation and proliferation were determined by nitro blue tetrazolium (NBT) dye reduction and immunocytochemistry of CD44 RPE cell surface antigen expression. Results: Transfection of c-met antisense oligonucleotides (2uM) reduced the expression of c-met mRNA by 50% to 65%. It also inhibited RPE cell proliferation and blocked CD44 expression. Conclusion: Antisense oligonucleotides effectively inhibit c-met expression, RPE cell differentiation and proliferation.

Keywords: 567 retinal pigment epithelium • 419 gene transfer/gene therapy • 523 proliferation 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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