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RK Zoltoski, RG Haiar, RA Trachimowicz, KJ Al-Ghoul, JR Kuszak; Structure/Function Analysis of Guinea Pig Lenses During Development, Growth and Age . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4632.
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Purpose: To correlate changes in lens dimensions and suture patterns with optical function (sharpness of focus) during defined periods of development, growth and age of the guinea pig. Methods: Lenses were collected from guinea pigs at various ages : newborn (n=9), 1 month (n=9), 3 months (n=9), 6 months (n=9), 12 months (n=8), and 24 months (n=10). The back vertex distance (BVD) and variability in BVD were assessed using a ScantoxTM In Vitro Assay System. Lenses were then measured, and suture patterns were documented. Data are presented as avg. ± s.e.m. Non-parametric statistics were used to assess differences between age groups. Groups were compared to the next youngest group (i.e. the 3 month group was compared to the 1 month group). Results: Lens width (equatorial axis) increased significantly throughout the first 6 months of life, while lens thickness (antero-posterior axis) only increased significantly over the first 3 months. A significant increase in average BVD during the first month corresponded to the largest increase in posterior radius. However, variability in BVD decreased significantly by the third month. This improvement in focus corresponded to the formation of a four branch "X" suture pattern overlain onto the 3 branch Y suture pattern formed throughout embryonic development. There were no significant changes in size, shape or optical properties between adult (12 month) and mid aged (24 month) groups. Sharpness of focus in mid aged lenses (12-24 months) was consistent with the evolution of a third generation of sutures (six branch "simple star") being overlain onto the 4 branch X suture. View OriginalDownload SlideView OriginalDownload Slide Conclusion: Differences in BVD were indicative of changes in the shape of the lens. The consistent level of sharpness of focus (average variability in BVD) was directly related to discontinuous or offset suture patterns. This is the first quantitative demonstration of evolving sutural complexity in a non-primate mammalian lens.
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