December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Viscoelastic Properties Of The Porcine Beta-heavy Lens Crystallins Using Dynamic Light Scattering
Author Affiliations & Notes
  • A Mitra
    Department of Chemical Engineering Washington University Saint Louis MO
  • E Remsen
    Washington University St Louis MO
    Department of Chemistry
  • P Hamilton
    VA Medical Center
    Washington University St Louis MO
  • N Ravi
    Department of Ophthalmology and Visual Sciences School of Medicine
    Washington University St Louis MO
  • Footnotes
    Commercial Relationships   A. Mitra, None; E. Remsen, None; P. Hamilton, None; N. Ravi, None. Grant Identification: Support: VA Merit Review Grant to N.R.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4653. doi:
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      A Mitra, E Remsen, P Hamilton, N Ravi; Viscoelastic Properties Of The Porcine Beta-heavy Lens Crystallins Using Dynamic Light Scattering . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4653.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To better understand the pathophysiology of presbyopia and cataract formation, the viscoelastic properties of the components of the natural lens need to be evaluated. In this study, the viscoelastic properties of the porcine beta-heavy lens crystallins are determined using Dynamic Light Scattering (DLS). Methods: The relaxation time spectra of porcine beta-heavy lens crystallins was obtained using DLS (Temperature 27ºC, λ = 514.5nm, scattering angle 90º) by Laplace inversion of the time-averaged intensity autocorrelation function. The relaxation time peaks obtained using Exponential sampling, Non-Negative Least Squares (NNLS) method, regularization programs CONTIN and REPES were compared. Results: The overall relaxation time distribution obtained from comparison of the above methods was evaluated as a function of concentration. Using REPES, it was seen that as the beta-heavy lens crystallins concentration increases: (a) The number of slow diffusion modes increases and extends over longer range of delay times, (b) The relative amplitude of the fast mode decreases. The hydrodynamic radius was calculated using the Stokes-Einstein relation and solution viscosity. Conclusion: With increasing beta-heavy lens crystallins concentration, the ratio of the relative amplitude of the fast or transient network mode to the slow modes decreases, indicating that cluster formation dominates the distribution at high concentrations. Comparison of the decay time spectrum obtained with DLS and mechanical measurements as well as angular dependence measurements are being investigated. Overall Relaxation Time Distribution as a function of Concentration  

Keywords: 378 crystallins • 526 protein purification and characterization • 338 cataract 

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