December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Determination of Modifications in Three Isoforms of a 9 kDa Gamma D-Crystallin Fragment Isolated from Human Lenses
Author Affiliations & Notes
  • K Srivastava
    Physiological Optics University of Alabama at Birmingham Birmingham AL
  • OP Srivastava
    Physiological Optics University of Alabama at Birmingham Birmingham AL
  • Footnotes
    Commercial Relationships   K. Srivastava, None; O.P. Srivastava, None. Grant Identification: NIH EY06400 and Retirement Research Foundation
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 4659. doi:
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      K Srivastava, OP Srivastava; Determination of Modifications in Three Isoforms of a 9 kDa Gamma D-Crystallin Fragment Isolated from Human Lenses . Invest. Ophthalmol. Vis. Sci. 2002;43(13):4659.

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Abstract

Abstract: : Purpose: The aim of the study was to characterize post-translational modifications in the three isoforms of a 9 kDa γD-crystallin fragment. Method: The three isoforms of a 9 kDa γD-crystallin fragment were isolated as previously described (Exp. Eye Res.62, 593-604, 1996), and were further separated by two-dimensional gel elctrophoresis. Individual isoforms were excised from 2D-gels and digested with either trypsin or chymotrypsin and analyzed by mass spectrometry. Results: By mass spectrometric analysis, the 9 kDa isoforms I, II and III showed Mr of 11033, 10903 and 10868, respectively. On chymotrysin digestion followed by mass spectrometric analyses of the isoforms, a fragment with a mass of 1374 (representing residue no. 154 to 166 of γD with a sequence of QDWGATNARVGSL) was detected in the isoform III but was at very low levels or almost non-existent in the isoforms I and II. A similar mass spectrometric analysis following trysin digestion of the isoforms, showed that W at position 156 was modified and M at position 146 was oxidized. Conclusion: The results show a potential modification region of residue no. 154-166 in the three isoforms of γD-crystallin fragment in human lenses. Two residues, M and W at positions 146 and 156, respectively, were modified in all the three isoforms.

Keywords: 378 crystallins • 525 protein modifications-post translational • 527 protein structure/function 
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