Human CM cell lines (CM2005.1, CRMM1, and CRMM2) and UM cell lines (Mel270, Mel290, and OM431) were used for the experiments. Cell line CM2005.1 was isolated by de Keizer et al.,
35 CRMM1 and CRMM2 were isolated by Nareyeck et al.,
36 Mel270 and Mel290 were isolated by Chen et al.
37 and Verbik et al.,
38 and OM431 was isolated by Albert et al.
39 All CM cell lines used for the present work were kindly provided by one of the authors (MJJ), and all UM cell lines were kindly provided by Bruce R. Ksander (Schepens Eye Research Institute, Boston, MA, USA). Cell line CM2005.1 derived from a primary tumor originally located at the palpebral conjunctiva of the upper eyelid, and was isolated from a local recurrence appearing 3 years after initial tumor removal.
35 The tumor displayed a spindle-cell type,
40 and immunohistochemical staining was positive for melanocytic markers S-100, MelanA, and HMB 45.
35 Cell line CRMM1 derived from a tumor originally located in the bulbar conjunctiva and was isolated from a local recurrence.
36 Cell line CRMM1 showed a high expression of tumor marker HMB-45 and displayed spindle-cell morphology.
40 Also, CRMM2 cells derived from a tumor originally located at the bulbar conjunctiva and was isolated from a recurrent tumor
36 showing high HMB-45 expression and displaying a spindle-cell type.
40 All tested CM cell lines were shown to have either BRAF (CRMM1, CM2005.1) or NRAS (CRMM2) mutations.
40