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Hongfei Ye, Yongxiang Jiang, Qinghe Jing, Dan Li, Tuerhongjiang Maimaiti, Dilinuer Kasimu, Yi Lu; LOXL1 Hypermethylation in Pseudoexfoliation Syndrome in the Uighur Population. Invest. Ophthalmol. Vis. Sci. 2015;56(10):5838-5843. doi: 10.1167/iovs.15-16618.
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© ARVO (1962-2015); The Authors (2016-present)
High prevalence of pseudoexfoliation syndrome (PEX) occurs in the Uighur population. This study investigated DNA methylation of the lysyloxidase-like 1 (LOXL1) gene in Uighur PEX patients with cataracts.
The research involved 10 lens capsule specimens from Uighur PEX patients with cataracts and 10 lens capsule specimens from Uighur control patients with age-related cataract (ARC) alone. All specimens were freshly collected during cataract surgery. Methylation status of the CpG islands was analyzed using pyrosequencing. The mRNA levels of LOXL1 were evaluated by quantitative real-time PCR, and protein levels were evaluated by Western blot assay.
For all the six chosen CpG islands of the LOXL1 gene promoter, hypermethylation was found in the PEX with cataracts compared to the age-matched ARC group. At the same time, the expression level of LOXL1 mRNA was significantly reduced in the PEX with cataracts group than that in the ARC group, and the expression level of the LOXL1 protein product demonstrated a similar tendency.
The susceptible PEX gene LOXL1 undergoes DNA hypermethylation in its promoter region in Uighur PEX with cataracts patients. This indicates that epigenetic regulation might play roles in PEX pathogenesis.
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