The RNA was isolated from RPE cells or induced T-cell subsets with TRIzol and were reverse transcribed with PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Dalian, China). Real-time quantitative PCR (RT-qPCR) was performed with SYBR Premix Ex Taq (TaKaLa) on 7900HT Fast Real Time PCR System (Applied Biosystems, Carlsbad, CA, USA). Mouse PCR primers sequences are as follows:
IL-17A, 5′-CCTGGCGGCTACAGTGAAG-3′, 5′-TTTGGACAC GCTGAGCTTTG-3′; RORγt, 5′-CCTCAGCGCCCTGTGTTTT-3′, 5′-GAGAACCAGGGCCGTGTAGA-3′; T-bet, 5′-CAACAACCCCTT TGCCAAAG-3′, 5′-TCCCCCAAGCAGTTGACAGT-3′; IFN-γ, 5′-GCTCTGAGACAATGAACGCT-3′, 5′-AAAGAGATAATCTGGCTC TGC-3′; IL-1β, 5′-AGTTGACGGACCCCAAAAGAT-3′, 5′-GTTGATGTGCTGCTGCGAGA-3′; IL-12b, 5′-CCATCGTTTTGC TGGTGTCTC-3′, 5′-GTCATCTTCTTCAGGCGTGTCA-3′; IL-6, 5′-CAGTTGCCTTCTTGGGACTGA-3′, 5′-TTGCCATTGCACAAC TCTTTTC-3′; IL-18, 5′-CAGGCCTGACATCTTCTGCAA-3′, 5′-TCTGACATGGCAGCCATTGT-3′; VEGFa, 5′-GGTGGACATCTTC CAGGAGT-3′, 5′-TGATCTGCATGGTGATGTTG-3′; COX2, 5′-TGCTCACGAAGGAACTCAGC-3′, 5′-CTCATACATTCCCCACGG TTTTG-3′; NLRP3, 5′-GAGTTCTTCGCTGCTATGT-3′, 5′-ACCTTCACGTCTCGGTTC-3′; IL-10, 5′-TCGGCCAGAGCCACA TG-3′, 5′-TTAAGGAGTCGGTTAGCAAGTATGTTG-3′; GATA3, 5′-AGAACCGGCCCCTTATGAA-3′, 5′-AGTTCGCGCAGGATGTC C-3′. Human PCR primer sequences are as follows: IL-1β, 5′-CATCAGCACCTCTCAAGCAG-3′, 5′-GAGTCCACATTCAGCACA GG-3′; IL-12b, 5′-ACCTGACCCACCCAAGAACT-3′, 5′-GGACCT GAACGCAGAATGTC-3′; IL-18, 5′-AGTCAGCAAGGAATTGTCTC C-3′, 5′-GAAGCGATCTGGAAGGTCTG-3′; VEGFa, 5′-TTCTGAG TTGCCCAGGAGAC-3′, 5′-TGGTTTCAATGGTGTGAGGA-3′; NLRP3, 5′-ACATCTCCTTGGTCCTCAGC-3′, 5′-GCTTCAGTCCC ACACACAGA-3′.