The mirVana miRNA-124 mimic, miRNA negative control (NC) mimic, mirVana miR-124 inhibitor, and miRNA NC inhibitor were purchased from Life Technologies (Carlsbad, CA, USA). The AccuTarget Fluorescein-labeled miRNA NC mimic was purchased from Bioneer (Daejeon, Korea). The NC small interfering RNA (siRNA) and predesigned RHOG-specific siRNA were purchased from Qiagen (Venlo, Limburg, The Netherlands). The Hs_miR-124a_1 miScript Primer Assay, Hs_RNU6-2_11 miScript Primer Assay, Hs_miR-16_2 miScript Primer Assay, miScript II Reverse Transcriptase, and QuantiTect SYBR Green PCR Master Mix also were obtained from Qiagen. Alexa Fluor 488-conjugated phalloidin and the Lipofectamine RNAiMAX and Lipofectamine 3000 transfection reagents were purchased from Life Technologies. Cellmatrix Collagen gel solution was obtained from Nitta Gelatin (Osaka, Japan). The following primary antibodies were used: anti-RHOG (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-fibronectin (Santa Cruz Biotechnology), anti-vimentin (Santa Cruz Biotechnology), anti–α-smooth muscle actin (SMA; Santa Cruz Biotechnology), anti-RAC1 (EMD Millipore, Billerica, MA, USA), anti-zonula occludens 1 (ZO-1; Abcam, Cambridge, UK), anti-occludin (Santa Cruz Biotechnology), and anti-GAPDH (Cell Signaling Technology, Danvers, MA, USA). All other chemicals were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA).