Erratum

Erratum in: “Contribution of TNF-α to Leukocyte Adhesion, Vascular Leakage, and Apoptotic Cell Death in Endotoxin-Induced Uveitis In Vivo” by Kan Koizumi, Vassiliki Poulaki, Sven Doehmen, Gerhard Welsandt, Sven Radetzky, Alexandra Lappas, Norbert Kociok, Bernd Kirchhof, and Antonia M. Joussen (Invest Ophthalmol Vis Sci. 2003;44:2184–2191) doi:10.1167/iovs.02-0589 

It was brought to the attention of the authors that bands demonstrating caspase-3 enzymatic activity in rat retinas in Figure 8A could have been assembled from different blots. The authors have now repeated the Western blot analysis. The new figure below repeats the results and should replace Figure 8A. 

Figure 8

 

(A) Western blot analysis for caspase-3. Each lane was loaded with equal amounts of protein. The anti-caspase-3 antibody (#9962; Cell Signaling Technology, Danvers, MA, USA) detects both procaspase-3 (35 KD) and the activated cleaved caspase-3 (17 KD) in the same lane. The band intensities of both bands were determined by densitometry of three Western blots as “adjusted volume” by ImageJ (http://imagej.nih.gov/ij/; provided in the public domain by the National Institutes of Health, Bethesda, MD, USA). The activity of caspase-3 was calculated as percentage of cleaved caspase-3 intensity to total caspase-3 intensity (sum of procaspase-3 and cleaved caspase-3) and is depicted in the added box plot adjusted to the controls. After lipopolysaccharide injection, caspase-3 protein levels were increased. The active (cleaved) form of caspase-3 was reduced after treatment with etanercept (P = 0.0294). Error bars: represent SEM (n = 3–6).

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