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Xinyi Su, Queenie S. W. Tan, Bhav H. Parikh, Alison Tan, Milan N. Mehta, Yeo Sia Wey, Sai Bo Bo Tun, Ling-Jun Li, Xiao-Yan Han, Tien Y. Wong, Walter Hunziker, Chi D. Luu, Yuji Owada, Veluchamy A. Barathi, Samuel S. Zhang, Shyam S. Chaurasia; Characterization of Fatty Acid Binding Protein 7 (FABP7) in the Murine Retina. Invest. Ophthalmol. Vis. Sci. 2016;57(7):3397-3408. doi: https://doi.org/10.1167/iovs.15-18542.
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To characterize the mouse retina lacking fatty acid binding protein (FABP7−/−).
Immunohistochemistry (IHC) was performed in 8-week-old mice to localize FABP7 in the retina. Retinal thickness was measured using image-guided spectral-domain optical coherence topography images. Electroretinography was carried out to assess retinal function. Fundus photography and fundus fluorescein angiography were performed on FABP7−/− and littermate wild-type (WT) mice, and retinal vascular changes were calculated using Singapore I Vessel Assessment (SIVA) analysis. Blood glucose levels were measured in the 8-week-old WT and FABP7−/− mice. In addition, retina was processed for trypsin digestion and retinal flat mounts for isolectin staining. Transcript levels of FABP7, VEGF, GFAP, and Na+K+ATPase were quantified using real-time PCR, and protein expression was analyzed by IHC and Western blot.
Fatty acid binding protein 7 is expressed in the inner nuclear layer, outer plexiform layer, and photoreceptor inner segments. No significant difference in retinal thickness and ERG responses was observed between FABP7-deficient and WT retinas. FABP7−/− mice have significantly decreased retinal venular caliber retinal arteriolar fractal dimension compared with WT littermates. FABP7−/− mice showed significant increased areas of fluorescein leakage in the retina. FABP7−/− mice exhibited elevated high blood glucose levels compared with WT mice. Trypsin digested FABP7−/− mice retina showed increased acellular strands and endothelial cell drop outs, and reduced microvasculature branching compared with WT retina. FABP7−/− mice retina also have increased GFAP and VEGF expression.
Fatty acid binding protein 7 is expressed in the retina and might play an important role in maintaining retinal vasculature.
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