Cell culture supernatant was harvested from cHCECs and centrifuged at 1580g at room temperature for 10 minutes to remove detached cells. Culture supernatant was collected and filtered through 0.220-μm filters (Millex-GV; EMD Millipore Corporation, Temecula, CA, USA). ELISA was performed by use of a Clusterin (Human) Competitive ELISA Kits (AdipoGen Corporation, San Diego, CA, USA), Procollagen type I C-peptide (PIP) EIA kit (TAKARA Bio, Inc., Kusatsu, Shiga, Japan), Human CCL2/MCP-1, Human TGF-β1, Human TIMP-1, and Human HGF (R&D Systems, Inc., Minneapolis, MN, USA), BMP2 Human Elisa Kit, PDGF-BB Human ELISA Kit, IL6 Human ELISA Kit, and Human IL8 ELISA Kit (Abcam plc., Cambridge, UK), Human Osteopontin Assay Kit and Human Osteopontin N-Half Assay Kit (Immuno-Biological Laboratories Co., Ltd., Fujioka, Gunma, Japan), Enzyme-Linked Immunosorbent Assay Kit For Early Growth Response Protein 1 (USCN Life Science, Inc., Wuhan, China), Human IP-10 ELISA Kit, Human IL-1RA ELISA Kit, Human IFN-γ ELISA Kit, and TNF-α Human ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA), Human Collagen Type IIIα1 (COL3A1) ELISA kit, and Human Collagen Type VIIIα2 chain (COL8A2) ELISA kit (CUSABIO, Wuhan, China).