Human Tenon's fibroblasts were fixed for 15 minutes in 4% paraformaldehyde; permeabilized for 10 minutes in PBS plus 0.1% Triton X-100 (Sigma-Aldrich Corp.); and blocked for 30 minutes in 10% normal goat serum (Boster, Wuhan, China). Cells were then incubated with rabbit anti-FN (1:100, Boster), mouse anti-TSP1 (1:100, Abcam), mouse anti-proliferating cell nuclear antigen (anti-PCNA) (1:100, Abcam), rabbit anti-collagen I (1:100, Proteintech), or rabbit anti-collagen III (1:100, Proteintech) at 4 °C. Cytoskeleton remolding was evaluated by double staining with FITC-phalloidin (Sigma-Aldrich Corp.) and mouse anti-vimentin (1:100, CST). Secondary antibodies were anti-mouse (Alexa Fluor 488 conjugate, green, or Alexa Fluor 555 conjugate, red, CST) and anti-rabbit (Alexa Fluor 555 conjugate, red, CST). Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich Corp.). Photomicrographs were captured by an Axioplan-2 imaging microscope system (Carl Zeiss, Inc., Jena, Germany). Cytoskeleton rearrangement was quantified as the intensity of F-actin fluorescence as measured by LSM 510 Examiner software (Carl Zeiss, Inc.). Ten randomly selected photomicrographs were analyzed, and the results were normalized by cell number.