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Ana Martynova, Amir H Kashani, Rodrigo A Brant, Michael Koss, Danhong Zhu, David R Hinton, Dennis O Clegg, Mark S Humayun; SD-OCT Assessment of Retinal Structure After Subretinal Implantation of a Substrate-Adherent Monolayer of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4270. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To use spectral domain optical coherence tomography (SD-OCT) to assess retinal thickness (RT) after subretinal implantation of human embryonic stem-cell derived retinal pigment epithelium adherent to a parylene membrane (CPCB-RPE1).
Fourteen minipigs were randomized to receive subretinal implantation of CPCB-RPE1 (n=11) or subretinal bleb formation alone (sham; n=3). All minipigs underwent preoperative imaging, clinical examination and postoperative evaluation at ~1 week and ~1 month. Measurements of RT, sublayer thickness and morphology overlying the implant were compared with topographically similar regions pre-implantation. Fluorescein angiography was performed to evaluate retinal inflammation and choroidal neovascularization at the implantation site. Imaging results were compared to post-mortem histology.
All animals underwent successful subretinal placement of CPCB-RPE1. Mean RT was negatively correlated with eccentricity and significantly different for regions <5.0mm (RT=326±53μm) and >5.1mm (RT=259±32μm) from disc margin in control and implanted pigs (p=0.0006). Mean RT overlying the CPCB-RPE1 implant was not significantly different from pre-implantation (308±72μm vs 292±41μm; p=0.4356). There was no significant difference in sublayer thickness pre and post implant in the retinal nerve fiber layer (RNFL; 57±17μm vs 50±13μm, p=0.2831), ganglion cell layer (GCL; 12±3μm vs 13±5μm, p=0.2232), inner plexiform layer (IPL; 66±10μm vs 64±18μm, p=0.7452), inner nuclear layer (INL; 24±13μm vs 21±9μm, p=0.5871), outer plexiform layer (OPL; 25±11μm vs 18±3μm, p=0.2777). Reliable outer nuclear layer measurement was only possible in 5 cases due to lack of a clear RPE-POS boundary over the implant and OPL boundary. The ONL measured 55±6μm preop vs 28±14μm postop (p=0.0001). However, there was no significant difference in sublayer thickness when measuring IPL through ONL (IPL to POS: 107±10μm vs IPL to implant: 111±22μm, p=0.4820). Histology at 30 days demonstrated photoreceptor interdigitation with RPE overlying the implant.
Subretinal placement of CPCB-RPE1 does not adversely impact RT at 1 month. SD-OCT findings demonstrate morphological patterns in the outer retina which may be the early signs of RPE-POS reconstitution overlying the implant.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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