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Ana Taba Oguido, Antonio M B Casella, Waldiceu Verri Junior, Miriam Hohmann, Felipe Ribeiro; Naringenin inhibits corneal neovascularization by anti-inflammatory and anti-oxidant mechanisms. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3510.
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© ARVO (1962-2015); The Authors (2016-present)
Cornea neovascularization leads to a loss of its transparency and decline in vision. Inflammatory cells are involved in promoting angiogenesis by secreting pro angiogenic factors like vascular endothelial grown factors (VEGF) and other cytokines. Naringenin (4′, 5,7-trihydroxyflavanone) is a flavanone that has been considered an antioxidant, anti-inflammatory and anti angiogenic agent playing a role in the prevention of many diseases. We investigated the effect of eye drops containing narigenin in a corneal alkali injury model in mice.
Mice were divided into seven groups of five each. A control group without neovascular stimulus with and whithout naringerin eye drops, and an injured group (NaoH 1N), a treated group wich received isotonic solution (NaCL 0.9%) or progressive concentrations (0.01, 0.1, 1.0, and 10g/L) of narigenin eye drops (8µL). The treatment in the rigth eye of mice started 3 days before the injury procedure and continued for 7 days. The following were analyzed: neovasculariztion areas by biomicroscopic digital photography, myeloperoxidase (MPO) and n-acetyl-β-D glucosaminidase (NAG) activity, antioxidant acitivity (glutathione GSH, ferric reducing antioxidant power FRAP, ability to scavenge 2, 2'- Azinobis-(3-Ethylbenzothiazoline 6-Sulfonic acid ABTS) and IL-1β, IL-6 and IL-10 interleukines levels.
Naringenin was more effective in the concentration of 10g/L as determined by MOP and NAG activity, on the third and seventh days after the injury procedure. Naringenin 10g/L reduced signicantly (p<0.01) the biomicroscopical neovascularization area. The total antioxidant capacity of naringenin (10g/L) eye drops was determined by reduced GSH, FRAP and ABTS assay, and they were prevented by 10g/L naringerin, 2,4 and 6 hours after injured. Morever, 10g/L naringenin solution inhibited the production of cytokines IL-1β and IL-6, significantly (p<0.05) and prevented the reduction of anti-inflammatory cytokine IL-10, measured 2, 4, 6 hours after injured by alcali. The results indicated that there were anti-inflammatory and antioxidant effects with the local eye drop use of narigenin.
We demonstrated the inhibitory effect of naringenin on corneal neovascularization of alkali-injured mice through anti-inflammatory and anti-oxidant mechanisms. Narigenin eye drops seem to be a promising adjunctive treatment for inflammatory neovascularization.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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