September 2016
Volume 57, Issue 12
ARVO Annual Meeting Abstract  |   September 2016
Diabetes compromises pH control in the rat retina.
Author Affiliations & Notes
  • Andrey V Dmitriev
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Desmond Henderson
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
  • Robert A Linsenmeier
    Biomedical Engineering, Northwestern University, Evanston, Illinois, United States
    Neurobiology, Northwestern University, Evanston, Illinois, United States
  • Footnotes
    Commercial Relationships   Andrey Dmitriev, None; Desmond Henderson, None; Robert Linsenmeier, None
  • Footnotes
    Support  NIH Grant R01EY021165
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 108. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Andrey V Dmitriev, Desmond Henderson, Robert A Linsenmeier; Diabetes compromises pH control in the rat retina.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):108.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose : To determine whether the ability of the rat retina to control its pH is affected by diabetes.

Methods : Double-barreled H+-selective microelectrodes were used to measure local extracellular [H+] in the retina of dark-adapted anesthetized Long-Evans rats. Recordings were performed in control and diabetic rats 1 to 6 months after intraperitoneal injection of vehicle or streptozocin. The microelectrode penetrated to the choroid in steps and then was slowly withdrawn to generate a profile that characterized transretinal H+ distribution. Two manipulations – increasing of blood glucose and injection of carbonic anhydrase blocker dorzolamide (DZM) – were used to interfere with retinal pH regulation, and results of these disturbances in control and diabetic retinas were compared. Averaged data are presented as mean±s.e., n –number of H+-profiles.

Results : As we have shown previously diabetic retinas often, but not always, were more acidic than controls. For these experiments we intentionally selected diabetic animals with mildly affected retinal pH. Adding intravenous glucose in control animals elevated plasma glucose from 144.2±10.0 (n=21) to 486.2±13.0 (n=22) mg/dL, but had almost no effect on the amplitude of the H+-profiles in the retina (37.8±1.9 and 35.0±2.8 nM, respectively). In contrast, similar injections of glucose in diabetic rats significantly increased both the glucose level (from 333.6±12.6 to 554.3±12.1 mg/dL, n = 18 and 19), and the amplitude of the H+-profiles (from 31.4±1.7 to 49.9±4.9 nM). The spatial heterogeneity of the H+ distribution in diabetic retinas that have been described earlier was also enhanced by elevating blood glucose. Suppression of carbonic anhydrase by DZM dramatically increased the amplitude of H+-profiles in control (from 36.5±1.6 to 126.9±9.4 nM, n = 17 and 13) as well as in diabetic retinas (from 44.3±3.3 to 129.4±13.3nM, n = 30 and 24). However, the duration of DZM application had an opposite effect in control and diabetic retinas. In controls, the strongest effect was recorded within 10 min after the injection, and then the effect slightly decreased. In diabetics, the effect of DZM tended to increase with time, and after 2 hours could be 2-3 times larger than at the beginning.

Conclusions : The data indicate that during development of diabetes the control over retinal pH is partly compromised so that diabetics are less able to withstand conditions that perturb retinal pH.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.