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Hyemin Seong, In Young Chung, Jong Moon Park, Seongwook Seo, Sang Soo Kang; Resveratrol Reduces Ischemia/Reperfusion-induced Apoptosis through Let-7 Family in Retina. Invest. Ophthalmol. Vis. Sci. 2016;57(12):183. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Ischemia/reperfusion (I/R) injury induces neuronal damage leading to apoptosis. Resveratrol (Res) can regulate the microRNA (miRNA) let-7 family which is one of the well-known miRNA. The present study a the correlation between Res, let-7 family and caspase-3 in I/R induced retinal injury mouse model.
To determine the effects of Res and let-7 family on the I/R retinal injury, the C57BL/6 were injected with Res once daily for 1 week. After that, I/R retinal injury was induced by high intra-ocular pressure. We observed retinal morphology change by H&E staining. We measured the let-7 family-caspase-3 mRNA correlation, caspase-3 protein expression and apoptosis by real-time PCR, western blotting analysis and TUNEL assay in retina tissue.
The caspase-3 mRNA expression increased after I/R injury. Conversely, levels of let-7 decreased. In Res injected group, let-7 levels were rescued. The levels of caspase-3 mRNA and caspase-3 protein were suppressed. But, the levels of caspase-8 and caspase-9 mRNA were not changed. TUNEL-positive apoptotic cell death was also decreased.
Resveratrol reduces caspase-3 mediated apoptosis through the let-7 family upregualtion in I/R-induced retinal Injury model.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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