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Kyung-A Kim, Sung Jae Yang, Tae-Jin Kim, Suk Woo Kang, Sang Hoon Jung; Anti-inflammatory effects of Diospyros kaki in Mouse Dry Eye Model. Invest. Ophthalmol. Vis. Sci. 2016;57(12):408.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effects of ethanol extract of Diospyros kaki (EEDK) on dry eye in a murine model.
Dry eye model was experimentally induced by topical administration of benzalkonium chloride (BAC) in a mouse. EEDK was orally administered throughout the experimental period. Tear break-up time (BUT) test, fluorescein staining, phenol red thread test, and histological analysis were performed on the ocular surface. Apoptotic cell death was tested by in situ terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay The protein expression levels of cytokeratin-10, interleukin-1 alpha (IL-1α), IL-1β, IL-6, tumor necrosis factor- alpha (TNF-α) and monocyte chemotactic protein- 1 (MCP-1) was detected by western blotting. The cellular proliferation of corneal epithelial cells was evaluated by immunohistochemistry (IHC).
Oral administration of EEDK resulted in prolonged tear break-up time (BUT), decreased fluorescein score, increased tear volume, and smoother epithelial cells compared with BAC treatment alone in the cornea. Moreover, up-regulated inflammatory cytokines, elevated squamous epithelium cells, and increased apoptotic cells were inhibited by treatment with EEDK. Proliferative activity was improved in corneal epithelium cells of EEDK-treated mice.
EEDK has potential as a beneficial agent in the clinical treatment of dry eye.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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