Purchase this article with an account.
Chae Eun Kim, Jihyun Lee, So Yeon Choi, Min Chae Lee, Chan Hum Park, JaeWook Yang; Effects of silk fibroin in a dry eye mouse model. Invest. Ophthalmol. Vis. Sci. 2016;57(12):431.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigated the effects of silk fibroin on the cornea, conjunctiva, and lacrimal gland in a dry eye mouse model.
The experimental dry eye mouse model was subcutaneous injections of scopolamine (muscarinic receptor blocker) and exposure to an air draft at an ambient humidity of 30-40% for 18 hours per day for 10 days on the 12- to 16-week-old NOD.B10.H2b mice. Tear volume and corneal smoothness were measured at 3, 5, 7 and 10 days after the instillation of phosphate buffered saline (PBS group) or silk fibroin (Silk group; 1 or 5 mg/ml). The corneal detachment and conjunctival goblet cell density were sectioned and stained with hematoxylin and eosin (H&E) and periodic acid Schiff (PAS) in the cornea and conjunctiva. The expression of inflammatory markers (i.e., TNF-α, MMP-2, MMP-9, ICAM-1, and VCAM-1) was detected by immunohistochemistry in the lacrimal gland. All data were statistically processed using the SPSS version 18.0.
The Silk group (1 or 5 mg/ml), tear production was increased by up to 2.1-fold, and corneal smoothness was improved to 46.2% and 53.8%, respectively, compared to the PBS group (P < 0.05). The detachment of the corneal epithelial cells was reduced by 90.9% in the silk group compared to the PBS group, and the conjunctival goblet cell density was significantly recovered to 1.4-fold and 1.7-fold, respectively, compared to the PBS group (P < 0.05). The expression of inflammatory factors was decreased in the lacrimal gland of the silk group compared to the PBS group.
These results show that silk fibroin improved on the cornea, conjunctiva, and lacrimal gland in the dry eye mouse model. These findings suggest that silk fibroin has anti-inflammatory effects in experimental model of dry eye.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
This PDF is available to Subscribers Only