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Kyoungyul Seo, Sangchul Yoon, Soojung Han, Jahyun Song, Hong Kyung Kim, Ho Sung Jung; Standardization for mouse phenotype in various stain with optical coherent tomography (OCT) and electroretinography (ERG). Invest. Ophthalmol. Vis. Sci. 2016;57(12):642.
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© ARVO (1962-2015); The Authors (2016-present)
The mouse ophthalmic biometry is very important for reasearch into the fields of eye disease. However, the in vivo corneal and retinal phenotype was not standardized. We measrued in vivo thickness and morphology in cornea, lens and retina in five various strain using OCT and ERG and to suggest standard normative range.
Standardized protocols for the accuracy using OCT and ERG, while setting up a normative range of cornea and retina thickness in BALB/C, C57BL6/J, 129s, Ahnak and C3H-KIBB mice. (n=5 in each strain) In order to establish the mice morphological experimental standard protocol, we have evaluated depth of anesthesia, dryness of cornea, temperature of body, and intensity of light exposure. OCT images of cornea and retina were taken from five strains of mice from 6-week-old to 16-week-old in every two consecutive week to evaluate the normative range.
In the comparison anesthesia depth between 80% and 120%, the lens opacity existed less in low depth of anesthesia. (p=0.03) When cornea was wet enough, the lens opacity was lower. Intensity of light exposure might have increased the lens opacity. Cornea and retinal thickness were highly strain dependent. CCT exhibited continuous variation from 70.2 m in C57BL6/J to 140.8 m in BALB/C. Cornea thickness widely varied in BALB/C than other strains. Retinal thickness in the C3H-KIBB is lowest among five strain, it reflects on visial acuity using optokinetic drum. (p=0.001) Differences between the cornea and retinal thickness of males and females have been observed only in a few cases. Fundus photo reflected in characteristic of each strain, there were no yellow spot and crack founded in C57BL6/J.
Taken together, it is important to standardize experimental protocol and establishing normative data for each strain for the achievement in the mouse phenotype consortium that tries to explore pathophysiology of various eye diseases through morphologic phenomenon of mice since they provide fundamental backgrounds for future experiments.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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