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Larry L. David; Detection and quantitation of age-related post translational modifications in the human lens using high-resolution mass spectrometry. Invest. Ophthalmol. Vis. Sci. 2016;57(12):No Pagination Specified.
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© ARVO (1962-2015); The Authors (2016-present)
Presentation Description :
Post-translational modifications (PTMs) play an important role in both cellular function and pathology in the visual system. Due to the lack of crystallin turnover in mature fibers, the human lens provides ideal samples to detect and quantify age-related PTMs. Using the lens as a model system, specific examples of PTM detection and quantitation using liquid chromatography/mass spectrometry will be discussed, including the use of whole protein mass measurement, generation and interpretation of peptide MS/MS spectra, label free quantitation, quantitation using mass shifted and isobaric labels, and quantitation based on internal standards and targeted MS. These methodologies will be illustrated by studies of deamidation, glutathionylation, and cysteine oxidation/cross linking in the aged human lens. The importance of enrichment of PTM containing peptides in lens versus non-lens tissues will also be considered, as well as the role of high-resolution mass spectrometry.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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