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Kevin L Schey; Spatially-resolved proteomics by imaging mass spectrometry of ocular tissues. Invest. Ophthalmol. Vis. Sci. 2016;57(12):No Pagination Specified.
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© ARVO (1962-2015); The Authors (2016-present)
Presentation Description :
Proteomics methods have provided a comprehensive view of protein expression and modification in many tissues including specific ocular tissues; however, the location of protein expression within tissues remains an important, relatively unexplored area. Although immunohistochemical approaches can be used to define protein expression in tissues, this targeted approach requires specific antibodies that are often unavailable, particularly for modified proteins. Imaging mass spectrometry (IMS) produces images based on protein molecular weight and provides spatially-resolved information on hundreds to thousands of proteins in a single experiment. This presentation will describe experimental aspects of protein imaging by IMS including sample preparation, spatial resolution, and data analysis. In addition, specific examples of protein imaging in ocular tissues will be presented along with a view towards future capabilities.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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