September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Effects of Platelet-rich Plasma on Canine Corneal Fibroblasts in LPS induced Inflammatory Condition
Author Affiliations & Notes
  • Young Sam Kwon
    Department of Veterinary Surgery, Kyungpook National University, College of Veterinary Medicine, Daegu, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Young Sam Kwon, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1279. doi:
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      Young Sam Kwon; Effects of Platelet-rich Plasma on Canine Corneal Fibroblasts in LPS induced Inflammatory Condition. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1279.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : This experiment was performed to assess the anti-inflammatory and proliferative effects of platelet-rich plasma (PRP) on the canine corneal fibroblasts.

Methods : To examine PRP effects, the inflammation of corneal fibroblasts was induced by lipopolysaccharide (LPS). The groups were divided into three groups: control group, DMEM containing 10% fetal bovine serum; LPS group, culture medium with LPS-treated group; LPS-PRP group, culture medium with LPS and 5% PRP-treated group. The effect of PRP on the proliferation and migration was examined in the inflammatory in vitro condition. The mRNA expressions of the IL-1β, IL-6, IL-10, TNF-α, collagen type 1, MMP-9 and α-SMA were evaluated using RT-PCR.

Results : The 3% or more PRP groups significantly enhanced proliferation compared to control group in proliferation assay. The migratory capacity of canine corneal fibroblasts was stimulated with 5% or more PRP group. RT-PCR results showed significant higher levels of IL-6, collagen type I, MMP-9 and α-SMA compared to LPS group.

Conclusions : The results of this study suggest that application of PRP on canine corneal fibroblasts may reduce the LPS-induced inflammation and enhance corneal stromal matrix remodeling.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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