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Young Sam Kwon; Effects of Platelet-rich Plasma on Canine Corneal Fibroblasts in LPS induced Inflammatory Condition. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1279.
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© ARVO (1962-2015); The Authors (2016-present)
This experiment was performed to assess the anti-inflammatory and proliferative effects of platelet-rich plasma (PRP) on the canine corneal fibroblasts.
To examine PRP effects, the inflammation of corneal fibroblasts was induced by lipopolysaccharide (LPS). The groups were divided into three groups: control group, DMEM containing 10% fetal bovine serum; LPS group, culture medium with LPS-treated group; LPS-PRP group, culture medium with LPS and 5% PRP-treated group. The effect of PRP on the proliferation and migration was examined in the inflammatory in vitro condition. The mRNA expressions of the IL-1β, IL-6, IL-10, TNF-α, collagen type 1, MMP-9 and α-SMA were evaluated using RT-PCR.
The 3% or more PRP groups significantly enhanced proliferation compared to control group in proliferation assay. The migratory capacity of canine corneal fibroblasts was stimulated with 5% or more PRP group. RT-PCR results showed significant higher levels of IL-6, collagen type I, MMP-9 and α-SMA compared to LPS group.
The results of this study suggest that application of PRP on canine corneal fibroblasts may reduce the LPS-induced inflammation and enhance corneal stromal matrix remodeling.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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