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Gyeong Jin Kang, Tan Ngoc Truong, Eric Huang, Valerie Su, ShaoKui Ge, Lu Chen; Integrin alpha 9 blockade promotes corneal transplant survival. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1446.
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© ARVO (1962-2015); The Authors (2016-present)
The lymphatic pathway mediates corneal transplant rejection. We recently reported that corneal lymphatic vessels develop luminal valves as lymphangiogenesis proceeds. These valves express integrin alpha 9 (Itga-9) and play a critical role in directing lymph flow. This study was designed to investigate the effect of Itga-9 blockade on corneal transplant rejection.
Orthotopic corneal transplantation was performed between C57BL/6 (donor) and BALB/c (recipient) mice. The recipients were randomized to receive subconjunctival injections of either Itga-9 blocking antibody (kindly provided by Dr. Toshimitsu Uede, Hokkaido University) or isotype control twice a week for 8 weeks. Corneal grafts were evaluated by ophthalmic slit-lamp biomicroscopy and analyzed by Kaplan-Meier survival curve.
While Itga-9 blockade reduced lymphatic valvulogenesis associated with transplantation, this treatment promoted corneal graft survival, compared to the control condition.
Itga-9 is involved in corneal transplant rejection. Further study on this pathway may provide novel strategies to modulate lymphatic valvulogenesis and graft rejection.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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