September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Diquafosol ophthalmic solution increases pre- and post-lens tear film during contact lens wear in rabbit eyes
Author Affiliations & Notes
  • Shizuka Koh
    Ophthalmology, Osaka University Graduate School of Medicine, Suita, Japan
  • Yukiko Nagahara
    Ophthalmology, Osaka University Graduate School of Medicine, Suita, Japan
  • Yoshihiro Oshita
    Nara Research & Development center, Santen Pharmaceutical Co., Ikoma, Japan
  • Takashi Nagano
    Nara Research & Development center, Santen Pharmaceutical Co., Ikoma, Japan
  • Hidetoshi Mano
    Nara Research & Development center, Santen Pharmaceutical Co., Ikoma, Japan
  • Hitoshi Watanabe
    Ophthalmology, Osaka University Graduate School of Medicine, Suita, Japan
  • Kohji Nishida
    Ophthalmology, Osaka University Graduate School of Medicine, Suita, Japan
  • Footnotes
    Commercial Relationships   Shizuka Koh, Santen (R); Yukiko Nagahara, Santen (R); Yoshihiro Oshita, Santen (E); Takashi Nagano, Santen (E); Hidetoshi Mano, Santen (E); Hitoshi Watanabe, Santen (R); Kohji Nishida, Santen (R)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 1473. doi:
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      Shizuka Koh, Yukiko Nagahara, Yoshihiro Oshita, Takashi Nagano, Hidetoshi Mano, Hitoshi Watanabe, Kohji Nishida; Diquafosol ophthalmic solution increases pre- and post-lens tear film during contact lens wear in rabbit eyes. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1473.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Decreased tear volume is a change related to contact lens (CL) wear. Prolonged increases in pre-lens tear meniscus height after instillation of 3% diquafosol ophthalmic solution during CL wear was previously reported. However, the post-lens tear film dynamics remain unknown. The aim of the present study was to investigate the behaviors of pre-lens tear film (PLTF) and post-lens tear film (PoLTF) after instillation of diquafosol by using an experimental rabbit model of eyes with CL.

Methods : Cross-sectional anterior segment optical coherence tomographic images of the inferior mid-peripheral cornea were obtained at baseline, and 5, 15, 30, 60, 90, and 120 min after instillation of 3% diquafosol ophthalmic solution in 10 Japanese rabbits wearing CLs. From the obtained images, the area of the PLTF and PoLTF were calculated. Both 0.1% sodium hyaluronate ophthalmic solution and artificial tear solution were used as controls.

Results : Fluid accumulation in PoLTF was observed 5 min after instillation of diquafosol and reached a peak at 15 min, followed by gradual reduction up to 120 min. PoLTF after instillation of diquafosol significantly increased from the baseline at 15 and 30 min (both P < 0.01). No fluid accumulation in PoLTF was observed after instillation of sodium hyaluronate and artificial tear. The increase in PoLTF up to 60 min after instillation of diquafosol was significantly higher (P < 0.01) than that after instillation of sodium hyaluronate and artificial tear. PLTF also significantly increased from its baseline value 30 min after diquafosol instillation (P < 0.05). The increase in PLTF after diquafosol instillation was significantly higher (P < 0.01) at 15 and 30 min than after instillation of sodium hyaluronate and artificial tear.

Conclusions : Instillation of 3% diquafosol ophthalmic solution increased PLTF and PoLTF in the rabbit eyes with CLs. Therefore, it has potential as treatment option for CL-related dry eyes.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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