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Chuang Nie, Peng Du, hongwei zhao, xinshuai nan, Yuxin Li, Wenbin Wei, Lianna Hu, Balamurali Ambati, Zaicheng Sun, Ling Luo; An Efficient fundus fluorescein angiography (FFA) Agent : Ag@Oxides Nanoprisms. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1708. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Nano-materials have been used widely applied for medical research because of its small size in recent years, however it is a virgin area in the field of ophthalmology. We created a new Silver-oxides core-shell nanoprisms (Ag@oxides NPs) -Fluorescein isothiocyanate (FITC) that be excited the fluorescence signal by 488nm. The purpose of this study was to demonstrate Ag @oxides nanoprisms can be used as a new effective agent in fundus fluorescein angiography (FFA).
Ag NPs were synthesized using a seed-mediated procedure. Ag@oxides NPs were prepared via a simple sol-gel route. The thickness of the oxides shell can be precisely tuned from 1 to 15 nm through changing the reaction time and amount of oxides sol-gel precursor. Cytotoxicity of Ag NP were tested in OCM-1 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. OCM-1 cells were incubated with Ag@oxides NPs for 24 h and then imaged with the fluorescence microscope. Fundus of C57BL/6J mice were checked immediately by fluorescence fundus angiography after the tail vein injection of 0.3ml of Ag@oxides nanoprisms (100 μg mL-1).
We developed a facile method to synthesize Ag@oxides core-shell NPs with a tunable shell thickness through the simple sol-gel route. Ag@oxides NPs exhibits a good biocompatiblity and stability due to the existence of oxide shell layer. MTT results showed that the cells were well tolerated to Ag@oxides NPs. The cells incubated with Ag@oxides NPs displayed quite intense green under laser excitation at wavelengths of 488 nm. The fundus fluorescein angiography (FFA) showed retinal vessels and capillary clearly and the fluorescence in retina lasted for 20 min at least.
Ag@oxides NPs might be used as a new agent for fundus fluorescein angiography.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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