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Ji Yang, Qing-Feng Wang, Men Lin, Zi-Bing Jin; The Effect of Electrical Stimulation on Cell viability and Proliferation in 661W cells. Invest. Ophthalmol. Vis. Sci. 2016;57(12):1759.
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© ARVO (1962-2015); The Authors (2016-present)
Recent studies suggested Electrical stimulation had the ability to rescue retina function from retinal disease. However, the effect of electrical stimulation on photoreceptor cells is still poorly understood. The purpose of this study was to detect the effect of electrical stimulation on cell viability and proliferation in 661W cells. In addition, we tried to preliminarily explore mechanism of the effect on 661W cells after electrical stimulation.
We designed an experimental instrument, in vitro cell electrical stimulation instrument (ESI). The ESI is composed of following parts: a two-channel power supply (STG-4002), the connecting circuits and 6-well plate’s lid equipped with four parallel U-shaped electrodes. 661W cells were plated into the 6-well plate of ESI and cultivated for 24h. 661W cells were supplemented with serum-free medium. And we divided 661W cells into two groups. One was stimulated by 600mv while the other one was stimulated by 900mv for 24h.We detected cell viability and proliferation by Cell Counting Kit-8 and EdU. The changes of mRNA and protein level were determined by Quantitative real-time RT-PCR and western blot.
After electrical stimulation for 24h, the cell viability increased in the electrical stimulation group compared to the control group (P<0.001). The rate of proliferating cells increased significantly in the electrical stimulation group compared to the control group (P<0.001). And the brain derived neurotrophic factor (Bdnf) mRNA (P<0.01) and protein (P<0.001) level were found increased after electrical stimulation compared to the control group.
Our results suggest that electrical stimulation has the ability to promote 661W cells’ viability and proliferation. In addition, we found the effect of electrical stimulation on 661W cells may depend on increasing Bdnf expression.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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