Abstract
Purpose :
To investigate the effect of Radix Hedysari Polysaccharide(HPS) on the expression of TRAF6 and TRIF during endotoxin-induced uveitis.
Methods :
Animal model of acute anterior uveitis was established by a hind footpad injection of male Wistar rats with 200 μg Cholera vibrio lipopolysaccharide （LPS）. HPS (400 mg/kg) or an equivalent volume of phosphate-buffered saline (PBS) was injected footpad 1 h before the LPS induction. The clinical manifestation was observed and scored at 2h intervals using a slit microscope. The degree of inflammatory reaction was determined by routine histological examinations. Real-time RT-PCR was used to assess the effects of HPS on the expression of TRAF6 ,TRIF and NF-κB p65 mRNA. The protein expression levels of TRAF6 and TRIF were examined by western blot. The expression of TRAF6 and TRIF in iris ciliary body tissue was detected through immunofluorescence study .
Results :
HPS treatment produced therapeutic results by significantly reducing the clinical severity of EIU as well as inflammatory reaction in the eye. But the result of RT-PCR and western blot showed that HPS treatment induced the mRNA and protein expression of TRAF6. The immunofluorescence results showed that HPS treatment significantly induced the expression of TRAF6 in the iris ciliary body. The effect of HPS on the expression of TRIF has no statistical significance.
Conclusions :
HPS can restrain the intraocular inflammation reaction in EIU by inhibiting TRAF6 from degradation. TRIF and MyD88 independent signal pathway was not the crucial pathway of EIU in rats.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.