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Jay Jyh-Kuen Siak, Nobuyo Yawata, Anne Jansen, Samanthila Waduthantri, Anita Chan, Gemmy Cheung, Soon-Phaik Chee; Human Leukocyte Antigen Associations with Cytomegalovirus Anterior Uveitis. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2341. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Cytomegalovirus (CMV) infection is associated with recurrent acute or chronic anterior uveitis / endotheliitis in immunocompetent individuals, especially among Asians. Major Histocompatibility Complex Class I restricted CD8+ T-cell response represents one of the major immune response that controls CMV, and previous studies suggested that different human leukocyte antigen (HLA) class I alleles can confer different levels of CD8+T-cell protection against human CMV reactivation. We hypothesize that these individuals may harbor an impaired immune defense against CMV. Therefore, we compared HLA class I genotypes of immunocompetent Chinese individuals with CMV anterior uveitis against that of Chinese healthy controls without ocular inflammation to identify potential HLA allele associations.
Genomic DNA was isolated from venous blood samples of 13 immunocompetent Chinese individuals with aqueous humor PCR proven CMV anterior uveitis against that of 28 age-matched Chinese healthy controls without ocular inflammation. HLA class I A, B, C typing was performed by Next Generation Sequencing on a MiSeq using Conexio’s Capture HLATM protocol and Assign MPS (Conexio Genomics PTE LTD, Perth, Australia).
HLA-B*1502 is significantly associated with CMV anterior uveitis compared to controls (Frequencies 38.5% versus 7.1%, Odds Ratio 8.13, (95% Confidence Interval 1.32-50.21)). The other common alleles among our Chinese subjects with CMV anterior uveitis include A*1101 (61.5%), A*2402 (53.8%), B*5801 (38.5%), C*0801 (38.5%) and C*0302 (38.5%). These genotype frequencies are higher than the previous reported frequencies among Singapore Chinese population.
Our data suggested that certain HLA haplotypes such as HLA-B*1502 may be associated with CMV anterior uveitis. These alleles are dominant in Chinese populations compared to Caucasian populations. Further studies are needed to validate our observation.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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