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Ethel Beatriz Guinto Arcos, Victor Manuel Bautista, Sergio Ulises Pliego-Nava, Herlinda Mejia-Lopez; Chlamydia sp genotypes associated with inclusion conjunctivitis in patients of an Ophthalmology Center in Mexico City. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2351.
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© ARVO (1962-2015); The Authors (2016-present)
To identify the predominant genotypes of Chlamydia sp in inclusion conjunctivits in Mexican population
160 conjunctival samples from patients with clinical diagnosis of inclusion conjunctivitis were evaluated. Molecular diagnosis was performed by real time PCR for identification of Chlamydia sp, using amplification of a segment of gene 16S common in Chlamydia species. Genotype identification was made by automated sequencing of OmpA gene using the Big Dye Terminator Cycle Sequencing Ready kit (Applied Biosystems, Foster City, CA). The obtained sequences were compared with those reported in GenBank.
The parcial sequences of ompA gene were analyzed manually and genotypes were compared and aligned with the NCBI database sequences via BLAST. The identification of the predominant serotypes was achieved. The most frecuently found were D, E, F and G servovars. In our study, all strains of E serovars were new genotypicvariants of Chlamydia trachomatis similar to those found in genital samples, recently reported in the GenBank by Indian researchers .
Chlamydia sp. infections are the most common bacterial sexually transmitted diseases in the world. The presence of this disease in different areas throughout the globe and its various ocular manifestations demonstrates the bacteria's ability to transcend race, class and age. Given the important impact of Chlamydia on the world's population in a variety of ways, clinicians and epidemiologists must collaborate on the development of optimal therapies and early diagnosis. The identification of Chlamydia sp. serovars responsible for inclusion conjunctivitis will facilitate the understanding and characterization of strains of epidemiological importance in this population.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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