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Zsuzsanna Zita Orosz, Amir H. Shemirani, Helga Bárdos, Bence Nagy, Andrea Facskó, András Berta, Róza Ádány, László Muszbek; Cellular Factor XIII is Present in the Corneal Stroma. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2368.
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© ARVO (1962-2015); The Authors (2016-present)
Transglutaminases (TGs) are a family of enzymes that cross-link proteins by e(g-glutamyl)lysyl bonds and most of them have been implicated in the modulation of extracellular matrix. Here we investigated the presence of three TGs, keratinocyte TG (TG-1), tissue transglutaminase (TG-2) and the cellular form of blood coagulation factor XIII (cFXIII) in the corneal tissue.
Frozen sections of normal human cornea obtained from enucleated bulbus were stained for cFXIII, TG-1 and TG-2 using poly-, or monoclonal antibodies. Detection of cFXIII was also combined with labeling for CD11b, CD34, CD45, CD68 and CD163 using double immunofluorescent staining. FITC-labeled or biotinylated secondary antibodies with Texas red-labeled streptavidin were used for the visualization of immunoreactions. Western blot analysis was also performed on corneal stroma, and the expression of cFXIII mRNA was analyzed by real-time qPCR.
A significant part of keratocytes showed intensive staining for cFXIII, but not for TG-1 and TG-2. Neither epithelial nor endothelial cells were labeled by anti-cFXIII antibody. cFXIII positive keratocytes were unevenly distributed in the corneal stroma; they were abundant in the subepithelial tertile of stroma (120±10/visual field), while they were sparse (38±6/visual field) in the subendothelial tertile. cFXIII+ cells showed co-staining for CD34, however, a significant number of CD34+ cells were negative for cFXIII. CD34+ cells were evenly distributed throughout the stroma. Only a few cells were stained for CD11b and CD45, they were also labeled by anti-cFXIII antibody. No cell showed positivity for CD68 and CD163. cFXIII expression was confirmed by Western blotting, and real-time qPCR demonstrated the presence of cFXIII mRNA in the corneal stroma.
This is the first report demonstrating the presence of cFXIII in the cornea. A significant part of CD34+ keratocytes contained cFXIII, their transglutaminase activity might play a role in wound healing of the cornea. Further investigations are needed to explore the role of FXIII in corneal wound healing.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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