September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Immunohistochemical characterization of subretinal bands in proliferative vitreoretinopathy
Author Affiliations & Notes
  • Mohammed D. Alotaibi
    medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia
  • Azza Maktabi
    Pathology, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia
  • hind Alkatan
    King Saud University, Riyadh, Saudi Arabia
  • Deepak P Edward
    Pathology, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia
  • Igor Kozak
    King Khaled Eye Specialist Hospital, Retina, Saudi Arabia
  • Footnotes
    Commercial Relationships   Mohammed D. Alotaibi, None; Azza Maktabi, None; hind Alkatan, None; Deepak Edward, None; Igor Kozak, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 2425. doi:
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      Mohammed D. Alotaibi, Azza Maktabi, hind Alkatan, Deepak P Edward, Igor Kozak; Immunohistochemical characterization of subretinal bands in proliferative vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2425.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : To provide immunohistochemical characterization of subretinal bands removed during retinal surgery in eyes with proliferative vitreoretinopathy (PVR).

Methods : This study included eyes with the clinical diagnosis of PVR that underwent pars plana vitrectomy surgery during which the subretinal bands associated with retinal detachment was obtained. The subretinal bands were removed “en bloc” through a retinotomy using subretinal intraocular forceps. The excised tissue was sent for histopathologic analysis. Immunohistochemistry (IHC) was performed to confirm the cellular nature and components of these subretinal membranes. The IHC stains included, glial fibrillary acidic protein (GFAP), pancytokeratin, CD3, CD20, CD68 and CD34.

Results : Subretinal membranes from 7 eyes were included in the analysis. All cases had successful surgical outcomes with reattachment six months after surgery. The microscopic examination of the excised tissue demonstrated following constituents of subretinal membranes: retinal pigment epithelial (RPE) cells (7/7), avascular spindle cell plaques of RPE cells metaplasia that stained positively with pancytokeratin (7/7). Fragments of gliotic GFA- positive neural retina was adherent to the fibrous plaque (6 /7). Bruch’s membrane was identified in one specimen. CD 68 positive macrophages were seen in 5/7 specimens and 2/5 of these specimens showed silicon oil- laden macrophages. Rare CD3 positive cells were also noted in one specimen.

Conclusions : Removal of subretinal membranes is essential for successful reattachment of the retina. Subretinal bands in PVR are mainly composed of reactive avascular plaques of retinal pigment epithelial fibrous metaplasia and macrophage infiltration. The overlying gliotic retina or Bruch’s membrane are likely to be adherent to such plaques and might be inadvertently excised during removal of such membranes.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.


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