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Shinsuke Shibata, Naoko Shibata, Teppei Shibata, Naoki Tanimura, Hiroshi Sasaki, Eri Kubo; Age-related cataract and expression level of decorin in human eye: association with opacity severity. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2500.
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© ARVO (1962-2015); The Authors (2016-present)
In an earlier study, we showed that DCN (decorin proteoglycan) expression level was increased among lens epithelial cells (LECs) using a posterior capsule opacity (PCO) rat model, albeit the reason was unclear. In the present study, we examined expression levels of DCN in human cataractous aqueous humor and LECs.
Approval was granted by Institutional Review Board, Kanazawa Medical University. Subjects included 82 cataractous eyes examined at Kanazawa Medical University Hospital, Ishikawa, Japan, from April to September, 2015. Age at time of presentation, subtype and severity of opacity according to WHO cataract classification system were recorded. Concentrations of DCN protein in aqueous humor were determined using Human DCN ELISA. DCN mRNA expression level in LECs was determined using real-time RT PCR.
DCN was found in aqueous humor, but the concentration varied among subjects showing no association with age, cataract subtype or grade. Expression of DCN mRNA in LECs differed markedly among subjects; however, it was significantly increased in eyes with posterior capsular (PSC) cataract grade 2-3.
To the extent of the authors’ knowledge, this is the first report describing DCN secreted in human aqueous humor and increased levels of DCN in LECs of human patients with advanced posterior capsular cataract. DCN may be involved in progression of PSC cataract.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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