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Toshiaki Hirakata, Kanae Kobayashi-Takakuwa, Toshinari Funaki, Nobuyuki Ebihara, Akira Matsuda; Hyperexpression of fatty acid binding protein 5 in atopic cataract. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2511.
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© ARVO (1962-2015); The Authors (2016-present)
The molecular mechanism of atopic cataract formation is still ill defined. To clarify pathophysiology of atopic cataract, we carried out genome wide gene expression analysis.
Two anterior capsules were obtained from atopic cataract patients with subcapsular cataract formation. Total RNA was extracted and genome wide gene expression analysis was carried out using Agilent SurePrint Human Gene Expression array. Anterior capsular tissue obtained from senile cataract patients were used as control. To confirm the results of gene array experiments, realtime PCR analysis was performed using 7 anterior capsules from atopic cataract patients and 5 from control patients.
The results of genomewide gene expression analysis showed 12 genes are hyperexpressed (more than 5 folds) in the anterior capsule of atopic cataract patients compared to the control. Among the 12 genes, fatty acid binding protein 5 (FABP5) gene expression is increased (36 fold, compared to the controls) in the replicative samples by realtime PCR analysis.
FABP5 gene, which have essential roles for lipid metabolism, may have some relations to the pathophysiology of atopic cataract.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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