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li xiaoxia, Zheng Zhang, Qian Liu, Ningli Wang; Selective mitochondrial autophagy in retinal ganglion cells following short-term reduction in cerebrospinal fluid pressure. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2535.
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© ARVO (1962-2015); The Authors (2016-present)
Short-term lowering of reduction in cerebrospinal fluid pressure(CSFP) with a disturbance of both the orthograde and retrograde axonal transport. To explore what underlie the axonal transport deficit, we examined mitochondria in retinal ganglion cells in Sprague Dawley rat.
In experimental group the CSFP was reduced by continuous aspiration of cerebrospinal fluid, and a sham control group (with a trocar in cisterna magna without cerebrospinal fluid release) was set. Under electron microscopy autophagosomes were observed in retinal ganglion cells. Selective mitochondrial autophagy related cytokines in retinal ganglion cells(RGCs) were analysis by immunohistochemistry or immunofluorescence. And protein was isolated from retinas for analysis of LC3-I,LC3-II, and lysosome-associated membrane protein 1 (LAMP1) by Western blot.
The antophagosomes in ganglion cell were significantly increased, the autophagy related proteins LC3-I, LC3-II, LAMP1 were increased in retinal sections, and the protein expression changes were also certified by western blot in experimental group.
This present study showed that selective mitochondrial autophagy is activated in RGCs, which could be explain the disturbance of both the orthograde and retrograde axonal transport following short-term reduction in cerebrospinal fluid pressure.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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