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Hideaki Okumichi, Wakana Iwata, Satoshi Okimoto, Ji-Ae Ko, Yoshiaki Kiuchi; Effects of rho-associated protein kinase inhibitor Y-27632 on scarring formation after glaucoma filtration surgery. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2928.
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© ARVO (1962-2015); The Authors (2016-present)
Glaucoma filtration surgery usually fails because of post surgical scarring, a process in which fibroblasts play a prominent role. To elucidate the effects of rho-associated protein kinase (ROCK) inhibitor Y-27632 in post surgical scarring (fibrosis), we have now investigated the molecular mechanism with human tenon fibroblasts.
Human tenon fibroblasts were cultured with Y-27632 or various antiglaucoma drugs for indicated periods. After cultivation, we have prepared total RNA and protein samples from tenon fibroblasts. Using multiple RT-PCR array, we examined the factors respond to Y-27632. And, we have studied the expression of factor(s) of relating scarring formation using RT-PCR, immunoblot and immunofluorescence analysis. Also, we have examined the three-dimensional collagen gels cultivation for gel contraction by various antiglaucoma drugs.
Collagen gel contraction by tenon fibroblasts was blocked in the presence of Y-27632. In multiple RT-PCR array using fibrosis-related genes, the expression of MMP-3 was down-regulated in tenon fibroblasts by additional Y-27632. Furthermore, immunoblot and immunoflurorescence analysis revealed that the expression of fibrosis markers was down-regulated in the presence of Y-27632.
These results suggest that the ROCK inhibitor Y-27632 may block scarring formation with interaction MMP-3 after glaucoma surgery. And, it will be possible that ROCK inhibitors and MMP-3 may have potential to be developed for treatment of glaucoma and other ocular diseases.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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