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Takako Sugimoto, Hideki Chuman, Nobuhisa Nao-i; Vasodilating effect of Ripasudil Hydrochloride Hydrate on isolated rabbit posterior ciliary arteries. Invest. Ophthalmol. Vis. Sci. 2016;57(12):2998.
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© ARVO (1962-2015); The Authors (2016-present)
Ripasudil (K-115) is a Rho-kinase inhibitor and is an ocular hypotensive agent. Ripasudil also has a vasodilating effect. The optic disc blood flow is supplied by posterior ciliary arteries (PCAs). This study aimed to clarify the vasodilatory effect of exogenous Ripasudil on isolated PCAs.
Vascular ring segments were mounted on a double myograph system. After obtaining the maximal contraction following the administration of a high-K solution, different concentrations of Ripasudil (100 nM to 100 μM) were administrated. When a vasodilatory effect was observed, carboxy-2-phenyl-4,4,5,5,-tetramethyl-imidazoline-1-oxyl-3-oxide (carboxy-PTIO), a nitric oxide scavenger, or NG-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, were administered. All isometric force measurements are given as relative values compared with high-K–induced maximal contractions.
Ripasudil significantly relaxed high-K solution-induced contracted rabbit PCAs in a concentration dependent manner (100 nM [34.8%±3.5]; 1 μM [78.5±17.3]; 10 μM [91.68±9.0]; 100 μM [94.41±8.3]). Carboxy-PTIO (1 mM) or L-NAME (300 mM) did not inhibit Ripasudil-induced relaxation in rabbit PCAs.
Ripasudil has a nitric oxide independent vasodilatory effect on high K-induced contractions in isolated rabbit PCAs.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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