September 2016
Volume 57, Issue 12
Open Access
ARVO Annual Meeting Abstract  |   September 2016
Hyperreflective Retinal Spots in Diabetic Eyes with and without Diabetic Macular Edema: B-Scan and en-face Spectral Domain Optical Coherence Tomography Evaluation.
Author Affiliations & Notes
  • Edoardo Midena
    University of Padova, Padova, Italy
    GB Bietti Foundation, IRCCS, Roma, Italy
  • Silvia Bini
    University of Padova, Padova, Italy
  • Tommaso Torresin
    University of Padova, Padova, Italy
  • Ferdinando Martini
    University of Padova, Padova, Italy
  • Porzia Pucci
    University of Padova, Padova, Italy
  • Annarita Daniele
    University of Padova, Padova, Italy
  • Raffaele Parrozzani
    GB Bietti Foundation, IRCCS, Roma, Italy
  • Olympia Kotsafti
    GB Bietti Foundation, IRCCS, Roma, Italy
  • Stela Vujosevic
    University of Padova, Padova, Italy
  • Footnotes
    Commercial Relationships   Edoardo Midena, None; Silvia Bini, None; Tommaso Torresin, None; Ferdinando Martini, None; Porzia Pucci, None; Annarita Daniele, None; Raffaele Parrozzani, None; Olympia Kotsafti, None; Stela Vujosevic, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science September 2016, Vol.57, 3252. doi:
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      Edoardo Midena, Silvia Bini, Tommaso Torresin, Ferdinando Martini, Porzia Pucci, Annarita Daniele, Raffaele Parrozzani, Olympia Kotsafti, Stela Vujosevic; Hyperreflective Retinal Spots in Diabetic Eyes with and without Diabetic Macular Edema: B-Scan and en-face Spectral Domain Optical Coherence Tomography Evaluation.. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3252.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate hyperreflective retinal spots (HRS) in diabetics with and without macular edema (DME vs no DME) on linear B scans and corresponding en-face imaging obtained using spectral domain OCT (SD OCT).

Methods : A retrospective detailed evaluation of OCT images of 38 eyes/subjects (19 diabetics without DME and 19 with DME) was performed. A population of normal 19 subjects/eyes served as control. On B scan SD OCT the following characteristics of HRS were evaluated: location (inner retina: IR or outer retina: OR); size (<30μ or >30μ); reflectivity (similar to retinal nerve fiber layer: RNFL or to retinal pigment epithelium: RPE); presence/absence of back-shadowing. On en-face SD OCT: absence of vessel or any other lesion; presence of vessel or microaneurysm; presence of hard exudates (confirmed on fundus color photo) were recorded. All gradings, were performed twice by two graders in a masked fashion.

Results : HRS size <30μ, reflectivity similar to RNFL, absence of back-shadowing and location in both IR and OR (mainly in DME patients), were associated with absence of vessels or any other lesion on en-face imaging (p=0.0001, for each evaluation). Size >30μ, reflectivity similar to RPE, presence of back-shadowing and location in the OR were all associated with the presence of hard exudates on en-face image, (p<0.0001, for each evaluation). Multiple logistic regression analysis showed that HRS presence in the IR (p<0.0001), size >30μ (p=0.0075), reflectivity similar to RNFL (p=0.02) and presence of back-shadowing (p<0.0001) are directly associated with the presence of microaneurysms on en-face imaging. Intra-grader and inter-grader repeatability were excellent for all evaluations, for both B scans and en-face images.

Conclusions : HRS can be easily evaluated on OCT images in normals and diabetics, even with DME. HRS with specific characteristics may have different origin. This data may help to better understand the pathophysiology of HRS in diabetic eyes, even vs controls.

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.

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