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Roberto Sessa, Preethi Padmanaban, Stephanie Wan, Steve Shen, April Smith, Terry Yamaguchi, Richard A Lang, Lu Chen; Overexpression of monocyte-derived Wnt5a promotes corneal lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3535.
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© ARVO (1962-2015); The Authors (2016-present)
Lymphangiogenesis (LG) accompanies many corneal diseases after an inflammatory, infectious, immunogenic, or chemical insult. It is known that monocyte-derived cells play a key role in this process. This study was designed to investigate the specific role of monocyte-derived Wnt5a in mediating corneal LG.
Macrophage specific conditional gain-of-function (GOF) mice were generated by cross breeding between Csf1R-cre and Rosa26Wnt5a mice. Standard suture placement model was used to induce corneal inflammatory LG. Whole-mount corneas were immunostained for LYVE-1 and analyzed by NIH Image J software. Additionally, macrophages were isolated from peritoneal cavity, and CD11b and F4/80 staining cells were quantified by flow cytometric analysis.
Compared to control condition, inflammatory LG response in macrophage specific Wnt5a GOF mice was enhanced, and the percentage of peripheral CD11b+/F4/80+ macrophages was increased as well.
Wnt5a promotes corneal LG. Further investigation on this pathway may offer novel insights and strategies to treat lymphatic disorders, which occur both inside and outside the eye.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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