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guangyu li, Ying Wen, liwei zhang, Lu Chen; RNA sequencing-based comparative transcriptome analysis of nasal-temporal difference of corneal lymphangiogenesis. Invest. Ophthalmol. Vis. Sci. 2016;57(12):3536. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Corneal lymphangiogenesis accompanies many disorders such as inflammation and transplant rejection. We have recently reported that this event occurs more predominantly at the nasal than the temporal side of the cornea. This study was to further investigate the mechanisms underlying this phenomenon.
Corneal lymphangiogenesis was induced by transplantation. RNA sequencing technique was used to investigate RNA expression profiles of the nasal and temporal sides of the corneas. FDR at 0.1 was used to select the significantly changed genes of differential expression.
Compared with the temporal side, 175 genes were up-regulated and 193 genes were down-regulated at the nasal side. The differentially expressed genes were mainly clustered into immune response, eye development, cell adhesion, defense response, signal peptide, transmembrane region, and extracellular matrix, etc. Lymphatic genes, such as Prox-1, LYVE-1, VEGFR-3 and Ang-2, were expressed at higher levels at the nasal than the temporal side.
Lymphatic vessels are not evenly distributed in the cornea. This phenomenon may be related to the anatomical and functional properties of the cornea and it may be regulated by multiple genes. Further investigation holds the promise for divulging new mechanisms and therapeutic strategies for lymphatic related diseases.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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