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Nobuhiro Nagai, Yasuko Izumida, Hirokazu Kaji, Matsuhiko Nishizawa, Aya Katsuyama, Shinji Yamada, Toru Nakazawa, Mineo Kondo, Hiroko Terasaki, Yukihiko Mashima, Toshiaki Abe; Protective Effects of Sustained Unoprostone Release Device in Rhodopsin Pro347Leu Transgenic Rabbits. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4017.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effects of sustained transscleral unoprostone isopropyl (UNO) administration for rhodopsin Pro347Leu transgenic (TG) rabbits.
We made a susteined UNO-release device (URD) consisting of a reservoir, controlled-release cover, and drug formulation, which were made of photopolymeized poly(ethyleneglycol) dimethacrylates. UNO, a prostone and BK channel activator for antiglaucoma eyedrops, was loaded in the device (length, 10 mm; width, 3.6 mm; height, 0.7 mm) at a content of 2.85 mg UNO. Six-week-old TG rabbits received episcleral implantation of URD on the right eye for 40 weeks. Electroretinogram (ERG)s and optical coherence tomography (OCT) were recorded before and after URD-implantation during successive examination.
UNO release from the devices was confirmed both in vitro and in vivo for more than 24 weeks. The b-wave amplitudes of the photopic ERGs (-0.050, 0.950, 1.477, 2.000 log cds/m2) were significantly larger in the URD-treated eyes than those of placebo and non-treated eyes (P<0.05, Tukey test) during 4-32 weeks implantation. The a-wave amplitudes of photopic ERGs were also significantly larger in the URD-treated eyes than those of others at 8 and 20 weeks implantation. The b-wave amplitudes of the scotopic ERGs (-1.523, -0.523, 0.477, 1.477 log cds/m2) were significantly larger in the URD-treated eyes than those of others during 4-20 weeks implantation. The a-wave amplitudes of scotopic ERGs were significantly larger in the URD-treated eyes in some points than those of otheres. There was no siginificant difference in ERG amplitudes between placebo-treated and non-treated eyes. The thickness of the ONL measured by OCT was also significantly preserved in URD-treated eyes than those of others in some points. No side effects were observed.
The URD treatment could preserve the ERGs amplitudes and ONL thickness in TG rabbits as long as the URD continues to release UNO. The URD might be a promissing transscleral application.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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