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Yasser Helmy Mohamed, Masafumi Uematsu; Corneal changes with Latanoprost use. Invest. Ophthalmol. Vis. Sci. 2016;57(12):4383. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the corneal changes after usage of Latanoprost along different periods of time.
Male white Japanese rabbits (KBT Oriental, Tosu, Japan) were used in this study. We divided the animals into 6 groups: Three groups for Latanoprost exposure corneas (immediately after latanoprost exposure, after 24 hour of exposure, after one week of exposure) and three groups as control groups with Hank’s Balanced Salt Solution (HBSS) exposure. Corneal transepithelial electrical resistance (TER) and transmission electron microscopy (TEM), and scanning electron microscopy (SEM) examinations were done for all groups.
There was a significant decrease in the corneal TER after immediate exposure of the cornea to latanoprost and no significant changes in the corneal TER after exposure of the cornea to other two groups of latanoprost or control groups. In the first group of immediate Latanoprost exposure, some of the superficial cells of the corneal epithelium were damaged, exhibited lost or degenerated microvilli, lost their adhesions with adjacent cells, and cracks appeared between cells by SEM examination. Separation between epithelial cells was confirmed with TEM examination. The superficial cells of the corneas of the second and third groups of Latanoprost exposure and control groups were normal in appearance with normal microvilli and normal adhesions between cells.
Latanoprost has acute transient toxic effect on the epithelial cells of the cornea. The regenerative power of the corneal epithelium counteracts of this toxic effect within 24 hours.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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