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Ling Wang, Zhaoxia Huang, Xiaohui Hu, Yizhi Liu, Quan Dong Nguyen, David W Li; SUMOylation Regulates Vimentin Functions in Lens. Invest. Ophthalmol. Vis. Sci. 2016;57(12):No Pagination Specified.
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© ARVO (1962-2015); The Authors (2016-present)
Previous studies have shown that mutation of E151K in vimentin leads to cataractogenesis. However, the molecular mechanism remains large unknown. Here we demonstrate that E151K mutation leads to abnormal SUMOylation of vimentin and interferes its polymerization.
RT-PCR was used to clone the wild type vimentin cDNA. In vitro mutagenesis was used to create the E151K mutant cDNA. Expression constructs of both wild type and mutant cDNAs were introduced into lens epithelial cells, the sumoylation patterns were determined by Western blot analysis. Cell morphology and microfilament polymerization were observed with fluorescence imaging.
Wild type and mutant vimentin display differential SUMOylation patterns and thus functional difference.
These results provide explanation why E151K mutation leads to cataractogenesis. (Supported by Research Prevent Blindness, NSFC, Zhongshan Ophthalmic Center, and Chinese Scholarship Council)
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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