Purchase this article with an account.
Takashi Tachibana, Toshio Hisatomi, Shoji Notomi, Shunji Nakatake, Yusuke Murakami, Akihito Sengoku, Yasuhiro Ikeda, Shigeo Yoshida, Tatsuro Ishibashi, Koh-Hei Sonoda; Vitreous and subretinal fluid ATP concentrations in rhegmatogenous retinal detachment. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5376.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Adenosine triphosphate (ATP) plays an important role in cell energy metabolism. During the course of apoptosis, dying cells release ATP into extracellular space. We previously reported that extracellular ATP accelerates photoreceptor cell apoptosis in subretinal hemorrhage. However, role of ATP in vitreous is not fully understood. The purpose of this study is to determine the vitreous levels of ATP and its correlation with clinical data in rhegmatogenous retinal detachment (RRD).
Vitreous samples were obtained from 58 eyes of 58 patients with RRD (n = 41), macular hole (MH; n=13), and epiretinal membrane (ERM; n =14) during pars plana vitrectomy. In RRD patient, subretinal fluids were also corrected from retinal tear during vitrectomy. We measured ATP concentration using luciferin-luciferase assay.
Compared with MH and ERM patient, RRD patient have higher vitreous ATP concentration (RRD: 1.74ng/ml, MH: 0.11ng/ml, ERM: 0.065ng/ml, p < 0.005, respectively). In RRD patient, subretinal fluid contains higher ATP than vitreous fluid (vitreous fluid: 1.74ng/ml, subretinal fluid: 13.72ng/ml, p<0.005).
RRD patient have high ATP concentration in vitreous. High ATP concentration in vitreous and subretinal fluid of RRD may be associated with photoreceptor apoptotic cell death.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
This PDF is available to Subscribers Only