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Zhaohua Yu, Nooshin Talebizadeh, Martin Kronschläger, Per G Soderberg; Indirect temperature measurement in the lens with temperature induced light scattering. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5740.
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© ARVO (1962-2015); The Authors (2016-present)
To estimate the temperature in the lens indirectly from the measurement of temperature-induced light scattering increase, based on Arrhenius equation.
The lens was extracted from six-weeks-old albino Sprague-Dawley female rats and put into a temperature-controlled cuvette filled with balanced salt solution. Altogether 40 lenses were equally divided and exposed to four different temperature of 40, 48, 58 and 70 °C, respectively, for 5 minutes. The intensity of forward light scattering was recorded during exposure.
The light scattering increase as a function of exposure time between 20 and 100 s was fit to a linear model. The inclination coefficient for temperature 40, 48, 58 and 70 °C was expressed as CI(0.95), 5.8±2.5, 13.4±6.3, 10.8±3.5 and 13.4±3.7 s-1 ×10-4. Arrhenius equation implies that the natural logarithm of the inclination coefficient is linearly dependent on the inverse of temperature with the proportionality constant and the intercept. The proportionality constant and the intercept, estimated as CI(0.95), was -2.5±1.0 K ×103 and 0.8±3.2. The activation energy was calculated as CI(0.95), 21.1±17.9 kJ●mol-1.
The in vivo temperature achieved in the lens can be determined indirectly from the measurement of light scattering increase after a certain exposure time, since the proportionality constant and the intercept are known from the current experiment.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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