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Luiza Neves, Vinicius Lima, Sultan Aldrees, Jacqueline Coblentz, Silvin Bakalian, Miguel N Burnier; Spleen tyrosine kinase as a proto-oncogene in uveal melanoma. Invest. Ophthalmol. Vis. Sci. 2016;57(12):5902.
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Spleen tyrosine kinase (Syk), a non-receptor tyrosine kinase, has been associated with cancer progression by acting as a proto-oncogene in some tumors, such as retinoblastoma, squamous cell carcinoma of the head and neck and naso-pharyngeal carcinoma. Conversely, Syk has been identified as a tumor suppressor in breast cancer, gastric cancer, and skin melanoma. The role of Syk in uveal melanoma (UM) has yet to be investigated. In this study, Syk expression in UM cells and in normal melanocytes was compared to further elucidate the role of Syk in UM.
Fifty-seven age matched eyes (23 UM and 34 normal human eyes [NHE]) were obtained from Henry C. Witelson Ocular Pathology Laboratory. Syk expression was analyzed using automated immunohistochemical staining and semiquantitatively evaluated using an immunoreactive score (IRS, 0-4). The IRS was calculated by the addition of staining intensity (0-2) and extension (0-2). Finally, the IRS was compared between normal choroidal melanocytes from normal human eyes and eyes containing UM, and also UM tumor cells. One-way analysis of variance (ANOVA) and Student’s t-test were used for statistical analysis.
UM showed significantly higher IRS compared to normal melanocytes from UM eyes (P=0.0001) and also compared to normal melanocytes in NHE (P=0.001). No significant difference in Syk expression was identified between normal melanocytes from UM eyes and normal melanocytes from NHE.
In this study, higher expression of Syk in UM cells compared to normal melanocytes from eyes harboring UM and from NHE was demonstrated. These results may indicate that in UM Syk acts as an oncogene with higher expression in tumor cells. Finally, Syk may be a suitable target for new therapeutics in UM.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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