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Jesus J Pintor, Hanan Alkozi, Juan Sanchez-Naves, Maria Jesus Perez de Lara, Gonzalo Carracedo, Begoña Fonseca, Alejandro Martinez Aguila; Elevated intraocular pressure increases melatonin levels in the aqueous humour. Invest. Ophthalmol. Vis. Sci. 2016;57(12):6401.
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© ARVO (1962-2015); The Authors (2016-present)
To study the levels of melatonin in the aqueous humour of normotensive and hypertensive IOP patients and to compare them in an animal model of glaucoma.
37 eyes of 37 patients who underwent cataract surgery were included in the study and were divided into normotensive patients, with IOP below 21 mmHg (n=23) and hypertensive patients, with IOP > 21 mm Hg (n=14). Glaucomatous DBA/2J (n=6) and control C57BL/6J (n=6) mice presenting 3 and 15 months of age for each strain were also used. Human and mice aqueous humours were aspirated using a 30-gauge Rycrof cannula on a tuberculin syringe and further processed to quantify melatonin by HPLC analysis.
Melatonin levels in normotensive patients (IOP below 21 mm Hg) presented values of 33.14 ± 11.61 ng/mL (n=23), while hypertensive patients (IOP above 21 mm Hg) showed melatonin concentrations of 95.87 ± 28.23 ng/mL (n=14) (p<0.039). Glaucoma mice presented melatonin values of 0.44 ± 0.06 ng/mL (at 3 months of age, before the pathology starts) which raised to 1.45 ± 0.18 ng/mL (at 15 months of age, when the pathology is fully established and IOP is maximum) (n= 6, p<0.001). Control mice did not significantly modified melatonin concentrations between 3 and 15 months of age.
Glaucoma patients with high IOP present increased concentrations of melatonin in their aqueous humour compared to normotensive patients. This has been confirmed in a glaucomatous animal model in which it has been possible to see a correlation between the development of the pathology, with an increase in IOP, and a concomitant elevation of melatonin in the aqueous humour.
This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.
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