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Paolo Carpineto, Agbeanda Aharrh-Gnama, Vincenzo Ciciarelli, Enrico Borrelli, Francesco Petti, Raffaella Aloia, Alessia Lamolinara, Marta Di Nicola, Leonardo Mastropasqua; Subretinal Fluid Levels of Signal-Transduction Proteins and Apoptosis Molecules in Macula-Off Retinal Detachment Undergoing Scleral Buckle Surgery. Invest. Ophthalmol. Vis. Sci. 2016;57(15):6895-6901. doi: https://doi.org/10.1167/iovs.16-20372.
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To evaluate signal transduction and early apoptosis protein levels in subretinal fluid collected during scleral buckling surgery for macula-off rhegmatogenous retinal detachment (RRD). Our aim was to assess both their relation with RRD features and their influence on the posttreatment outcome.
Thirty-three eyes of 33 RRD patients scheduled for scleral buckle surgery were enrolled in the study. Undiluted subretinal fluid samples were collected during surgery and analyzed via magnetic bead–based immunoassay. All patients underwent a complete ophthalmologic evaluation at baseline and at each follow-up visit (months 1, 3, and 6). Moreover, both at baseline and at the postsurgery month 6 visit, the patients were tested by means of spectral-domain optical coherence tomography (SD-OCT) in order to evaluate the average ganglion cell–inner plexiform complex thickness, as well as the photoreceptor inner segment/outer segment junction status.
Patients' clinical features (retinal detachment size, detachment duration, and occurrence of proliferative vitreoretinopathy) were associated with several early apoptotic factors (caspase-8, caspase-9, and B-cell lymphoma 2 [Bcl-2]–associated death promoter [BAD]). Furthermore, both early apoptosis factors (caspase-8, Bcl-2, and p53) and signal-transduction proteins (ERK 1/2) were found to influence the postsurgery month 3 OCT characteristics.
Signal-transduction proteins and early apoptosis proteins are associated with different clinical features and postsurgery outcomes.
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