Anti-RPE polyclonal antibody denoted S58 (named to indicate the origin from sheep number 58) and monoclonal anti-C5 antibody were obtained from Allergan, Inc. (Irvine, CA, USA). Goat anti-ApoE was purchased from Calbiochem/EMD (1:8000 for Western blot, cat. no. 178479; San Diego, CA, USA). Rabbit anti-ApoE antibody (1:100 for immunostaining, cat. no. PA5-27088; Chicago, IL, USA) and rabbit anti-zonula occludens-1 (ZO-1) were purchased from Thermo Scientific, Inc. (Chicago, IL, USA). Anti-apolipoprotein A1 (ApoA1) antibody was purchased from Cell Signaling Technology (1:1000, cat. no. 3350; Beverly, MA, USA). Monoclonal anti-human C5b-9 clone aE11 was purchased from Dako (Carpinteria, CA, USA). Rabbit anti-fibronectin antibody was purchased from Abcam, Inc. (1:8000, cat. no. ab23750; Cambridge, MA, USA). C1q-depleted human serum (C1q-Dep), C6-depleted human serum (C6-Dep), and purified C6 protein were purchased from Quidel Corp. (San Diego, CA, USA). Recombinant ApoE human protein with N-terminus thioredoxin and polyhistidine (N-TRX.His) tags was purchased from Life Technologies (cat. no. 10817-H30E; Frederick, MD, USA). Protein G–purified normal sheep IgG was purchased from Molecular Innovations, Inc. (Novi, MI, USA). Cycloheximide (CHX), ammonium hydroxide (NH4OH), phalloidin (cat. no. P1951), and collagen type I were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). radioimmunoprecipitation assay (RIPA) lysis and extraction buffer (cat. no. 89900), GelCode blue stain reagent (cat. no. 24590), and In-Gel tryptic digestion kit (cat. no. 89871) were purchased from Thermo Scientific, Inc.